MICROWAVE STEINER METHOD FOR SPIROCHETES AND BACTERIA

被引：15

作者：

CHURUKIAN, CJ

GARVEY, W

机构：

[1] Department of Pathology, University of Rochester Medical Center, Rochester, NY, 14642

[2] Department of Histopathology, Children’s Hospital of Eastern Ontario, Ottawa, ON

来源：

JOURNAL OF HISTOTECHNOLOGY | 1990年 / 13卷 / 01期

关键词：

Bacteria; Microwave oven; Spirochetes; Steiner method;

D O I：

10.1179/his.1990.13.1.45

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A microwave version of Steiner's method for demonstrating spirochetes and bacteria is described. The method differs froi Steiner's method and its modifications in that the concentration of the uranyl nitrate and silver nitrate solutions was reduced from 1% to 0.5%. Decreasing the concentration of the uranvl nitrate solution reduces exposure to this radioactive substance. Uranyl nitrate sensitization, silver nitrate impregnation, and the reduction were performed by microwave irradiation at temperatures well below the boiling point of the solutions. The entire procedure took about ten minutes to perform with even, consistent silver impregnation of the organisms. (The J Histotechnol 13:45, 1990). © 1990 Maney Publishing.

引用

页码：45 / 47

页数：3

共 21 条

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