THE INFLUENCE OF INVITRO DEVELOPMENT UPON POSTTHAW SURVIVAL AND IMPLANTATION OF CRYOPRESERVED HUMAN BLASTOCYSTS

被引：44

作者：

HARTSHORNE, GM

ELDER, K

CROW, J

DYSON, H

EDWARDS, RG

机构：

[1] Bourn Hall Clinic, Bourn

来源：

HUMAN REPRODUCTION | 1991年 / 6卷 / 01期

关键词：

BLASTOCYST; CRYOPRESERVATION; VIABILITY; IMPLANTATION; ABORTION; FREEZING DAMAGE;

D O I：

10.1093/oxfordjournals.humrep.a137247

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Blastocysts from 198 patients were frozen using glycerol as cryoprotectant. No difference in the post-thaw survival of blastocysts or implantation rates was found between 177 patients (122 transfers) with all surplus embryos cultured to blastocysts before freezing and 20 patients (12 transfers) whose embryos were considered unsuitable for freezing during cleavage and were then frozen as blastocysts. Nineteen pregnancies were achieved, of which six aborted. Pre-freezing morphology was similar in blastocysts of patients in groups 1 and 2 and did not relate to their survival after cryopreservation. A significantly lower proportion with suspected damage after thawing was present among patients becoming pregnant after transfers of single blastocysts (P < 0.01) and implanting embryos were in general more expanded at the time of transfer. No differences were detected between blastocysts resulting in normal development and those leading to abortion. The developmental consequences of damage to human blastocysts are discussed.

引用

页码：136 / 141

页数：6

共 28 条

[1]

Buster J.E., Bustillo M., Rodi I.A., Cohen S.W., Hamilton M., Simon J.A., Thomeycroft I.H., Marshall J.R., Biologic and morphologic development of donated human ova recovered by nonsurgical uterine lavage, Am. J. Obstet. Gynecol, 15, pp. 211-217, (1985)

[2]

Cohen J., Simons R.F., Edwards R.G., Fehilly C.B., Fishel S.B., Pregnancies following the frozen storage of expanding human blastocysts, J. in Vitro, 2, pp. 59-64, (1985)

[3]

Dokras A., Sargent I.L., Ross C., Gardner R.L., Barlow D.H., Trophectoderm biopsy in human blastocysts. Hum, Reprod, 5, pp. 821-825, (1990)

[4]

Dyce J., George M.A., Goodall H., Fleming T.P., Do cells belonging to trophectoderm and inner cell mass in the mouse blastocyst maintain discrete lineages?, Development, 10, pp. 685-698, (1987)

[5]

Edwards R.G., Conception in the Human Female, Academic Press, London, 67, pp. 894-895, (1980)

[6]

Edwards R.G., Hollands P., New advances in human embryology: Implications of the preimplantation diagnosis of genetic disease. Hum, Reprod, 3, pp. 549-556, (1988)

[7]

Fehilly C.B., Cohen J., Simons R.F., Fishel S.B., Edwards R.G., Cryopreservation of cleaving embryos and expanded blastocysts in the human: A comparative study.Fertil, Steril, 44, pp. 638-644, (1985)

[8]

Fleming T.P., A quantitative analysis of cell allocation to trophectoderm and inner cell mass in the mouse blastocyst. Dev, Biol, 11, pp. 520-531, (1987)

[9]

Gardner R.L., Edwards R.G., Control of the sex ratio at full term in the rabbit by transferring sexed blastocysts, Nature, 21, pp. 346-348, (1968)

[10]

Hardy K., Handy Side A.H., Winston R.M.L., The human blastocyst: Cell number, death and allocation during late pre-implantation development in vitro, Development, 10, pp. 597-604, (1989)

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