ESTIMATION OF BIOTINYLATED LECTIN BY ISOELECTRIC-FOCUSING

被引：2

作者：

HARADA, H ^{[1
]}

KONDO, M ^{[1
]}

YAMAGUCHI, T ^{[1
]}

机构：

[1] SUGIYAMA CHEM & IND LAB,YOKOHAMA,JAPAN

来源：

ELECTROPHORESIS | 1990年 / 11卷 / 06期

关键词：

D O I：

10.1002/elps.1150110613

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Concanavalin A (Con A) was biotinylated to various degrees using N‐biotinyl‐ω‐aminocaproic‐acid‐N‐hydroxy succinimide ester as the biotinylation reagent, and then analyzed by isoelectric focusing using PhastGel IEF 3–9. The isoelectric points of biotinylated ConAs were found to decrease with increasing concentration of the biotinylation reagent. Analysis by isoelectric focusing followed by dot blotting clearly indicated that the biotinylated ConA with an isoelectric point lower than that of the original ConA by 2.2 ± 0.6 had the strongest binding activity for ovalbumin. Copyright © 1990 VCH Verlagsgesellschaft mbH

引用

页码：505 / 506

页数：2

共 6 条

[1]

BAYER EA, 1982, METHOD ENZYMOL, V83, P195

[2] LIGANDS FOR INSULIN-RECEPTOR ISOLATION [J].