EFFECTS OF POTASSIUM DEPOLARIZATION ON INTRACELLULAR COMPARTMENTALIZATION OF ATP IN CHOLINERGIC SYNAPTOSOMES ISOLATED FROM TORPEDO ELECTRIC ORGAN

被引:3
作者
SOLSONA, C
SALTO, C
YMBERN, A
机构
[1] Laboratori de Neurobiologia Cellular i Molecular, Departament de Biologia Cellular i Anatomia Patològica, Facultat de Medicina, Hospital de Bellvitge, Universitat de Barcelona
关键词
ATP COMPARTMENTALIZATION; CHOLINERGIC SYNAPTOSOME; (TORPEDO);
D O I
10.1016/0167-4889(91)90044-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
It is well known that acetylcholine (ACh) and ATP are co-stored and co-released in nerve terminals of the electric organ of Torpedo. Cholinergic synaptosomes were subjected to a cycle of freezing and thawing showing that ATP is distributed in two operational pools like those described for ACh. The bound pool is resistant to freezing and thawing, and it is presumably protected by membranes. When metabolically active ATP was prelabelled with [H-3]adenosine, 76% of the radioactivity was associated with the free pool of ATP. When the preparation was depolarized in a calcium containing medium, there was a decrease in the specific radioactivity of ATP in the free pool and an increase in the bound pool. These results reflect that the patterns of distribution of ACh and ATP, in this synaptosomal preparation, are similar in resting conditions and during K+ depolarization.
引用
收藏
页码:57 / 62
页数:6
相关论文
共 41 条
[1]   CHOLINE METABOLISM IN CEREBRAL-CORTEX OF GUINEA-PIGS - STABLE-BOUND ACETYLCHOLINE [J].
BARKER, LA ;
DOWDALL, MJ ;
WHITTAKER, VP .
BIOCHEMICAL JOURNAL, 1972, 130 (04) :1063-1080
[2]   RELEASE OF ADENOSINE TRIPHOSPHATE FROM FROG SKELETAL MUSCLE IN VITRO [J].
BOYD, IA ;
FORRESTER, T .
JOURNAL OF PHYSIOLOGY-LONDON, 1968, 199 (01) :115-+
[3]  
BRIDGER WA, 1983, CELL ATP, P4
[4]  
BURNSTOCK G, 1990, ANN NY ACAD SCI, V603, P1
[5]  
BURNSTOCK G, 1987, ENCY NEUROSCIENCE, P1015
[6]  
CORCORAN JJ, 1986, J NEUROCHEM, V47, P945
[7]   TURNOVER AND STORAGE OF NEWLY SYNTHESIZED ADENINE-NUCLEOTIDES IN BOVINE ADRENAL-MEDULLARY CELL-CULTURES [J].
CORCORAN, JJ ;
WILSON, SP ;
KIRSHNER, N .
JOURNAL OF NEUROCHEMISTRY, 1986, 46 (01) :151-160
[8]  
DOWDALL MJ, 1974, BIOCHEM J, V140, P1
[9]   ON THE MECHANISM OF ACETYLCHOLINE-RELEASE [J].
DUNANT, Y .
PROGRESS IN NEUROBIOLOGY, 1986, 26 (01) :55-92
[10]   ACETYLCHOLINE COMPARTMENTS IN STIMULATED ELECTRIC ORGAN OF TORPEDO-MARMORATA [J].
DUNANT, Y ;
ISRAEL, M ;
GAUTRON, J ;
LESBATS, B ;
MANARANCHE, R .
JOURNAL OF NEUROCHEMISTRY, 1972, 19 (08) :1987-+