MORPHOLOGICAL-CHANGES IN OLIGODENDROCYTES IN THE INTACT MOUSE OPTIC-NERVE FOLLOWING INTRAVITREAL INJECTION OF TUMOR-NECROSIS-FACTOR

被引:41
作者
BUTT, AM
JENKINS, HG
机构
[1] Sherrington School of Physiology, UMDS, St. Thoma's Hospital, London, SE1 7EH, Lambeth Palace Road
基金
英国惠康基金;
关键词
OLIGODENDROCYTE; MORPHOLOGY; MYELIN; TUMOR NECROSIS FACTOR; CYTOKINE; DEMYELINATION; MULTIPLE SCLEROSIS;
D O I
10.1016/0165-5728(94)90125-2
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Intracellular dye-injection was used to determine the whole-cell morphology of oligodendrocytes in intact optic nerves of mice following intravitreal injection of tumour necrosis factor-alpha (TNF alpha) or heat-inactivated TNF alpha to act as controls. Oligodendrocytes in control nerves had a stereotypic morphology, and provided an average of 20 axons with single internodal myelin segments of around 200 mu m internodal length. Oligodendrocytes with abnormal morphological features were identified 8-14 days following intravitreal injection of TNF alpha. Internodal myelin segments developed swellings along their lengths, became attenuated, and in extreme cases receded completely. This study provides a new insight into the process of demyelination, especially of the early stages which are not amenable with other techniques. Furthermore, it confirms that injection of TNF alpha into the vitreous, a fluid compartment of the CNS, instigates a sequence of events which results in oligodendrocyte disruption and demyelination. The mechanism by which intravitreally injected TNF alpha mediates these changes in optic nerve oligodendrocytes are yet unknown.
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页码:27 / 33
页数:7
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