CYTOKINE PRODUCTION IN THE MUCOSA OF PATIENTS WITH INFLAMMATORY BOWEL-DISEASE

被引：10

作者：

JONES, SC

HAIDAR, A

HAGAN, P

CRABTREE, JE

BANKS, RE

AXON, ATR

DIXON, MF

WHICHER, JT

机构：

[1] Centre for Digestive Diseases, Leeds General Infirmary, Leeds

[2] Department of Endocrinology, National Institute for Biological Standards and Controls, Potters Barr, Hertfordshire

[3] Department of Clinical Medicine, St James’s University Hospital, Leeds

[4] Institute for Cancer Studies, St James’s University Hospital, Leeds

来源：

EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY | 1993年 / 5卷 / 08期

关键词：

INTERLEUKIN-6; TUMOR NECROSIS FACTOR; INFLAMMATORY BOWEL DISEASE;

D O I：

10.1097/00042737-199308000-00009

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Objective: To investigate mucosal secretion of interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNF-alpha) in inflammatory bowel disease patients and controls. Design: Prospective study of patients recruited from the colonoscopy lists and the colitis clinic of a teaching hospital gastroenterology unit. Methods: Mucosal biopsies from patients with inflammatory bowel disease and controls were cultured for 24 h and secretion of IL-6 and TNF-alpha was determined by enzyme-linked immunosorbent assay. Results: Interleukin-6 and TNF-alpha production in vitro from patients with active ulcerative colitis was significantly greater than from patients with inactive ulcerative colitis (P<0.005), inflamed mucosa in patients with Crohn's disease (P<0.05) and controls (P<0.005). Interleukin-6 production from inactive ulcerative colitis biopsy specimens was also significantly greater than from controls (P<0.02). Interleukin-6 and TNF-alpha secretion by ulcerative colitis biopsy specimens correlated with both acute and chronic inflammatory cell infiltrate (P < 0.002) and surface epithelial degeneration of the mucosa (P < 0.001). Conclusions: Interleukin-6 and TNF-alpha are secreted locally by the inflamed colonic mucosa in ulcerative colitis and Crohn's disease. The higher concentration of IL-6 in the supernatants from ulcerative colitis biopsy specimens contrasts with circulating IL-6 concentrations, which are higher in patients with Crohn's disease.

引用

页码：607 / 612

页数：6

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