EFFECTS OF N-TERMINAL DELETIONS OF THE ESCHERICHIA-COLI PROTEIN FIS ON GROWTH-RATE, TRNA(2)(SER) EXPRESSION AND CELL MORPHOLOGY

被引:5
作者
SPAENYDEKKING, L
NILSSON, L
VONEULER, A
VANDEPUTTE, P
GOOSEN, N
机构
[1] LEIDEN UNIV,GORLAEUS LABS,INST CHEM,GENET MOLEC LAB,2300 RA LEIDEN,NETHERLANDS
[2] UPPSALA UNIV,DEPT MOLEC BIOL,BMC,S-75124 UPPSALA,SWEDEN
[3] UNIV UPPSALA,DEPT HUMAN ANAT,BMC,S-75123 UPPSALA,SWEDEN
来源
MOLECULAR & GENERAL GENETICS | 1995年 / 246卷 / 02期
关键词
ESCHERICHIA COLI; FIS; MUTANTS; TRANSCRIPTION; MINICELLS;
D O I
10.1007/BF00294690
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Escherichia coli Fis protein is known to be involved in a variety of processes, including the activation of stable RNA operons. In this paper we study the ability of a set of N-terminal Fis deletion mutants to stimulate transcription of the tRNA(2)(Ser) gene. The results indicate that the domain of the Fis protein containing residues 1-26 is not required for transcription activation. The Fis mutants that are still active in transcription stimulation can also complement the reduced growth rates of Fis(-) cells, suggesting that the same activating domain is involved in this phenomenon. In addition, we show that in fast growing cultures in the absence of an active Fis protein, minicells are formed. These minicells seem to arise from septum formation near the cell poles. Suppression of minicell formation by Fis also does not require the presence of the N-terminal domain of the protein.
引用
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页码:259 / 265
页数:7
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