RAPID, RELIABLE AND ECONOMICAL SILVER STAIN FOR NEUROFIBRILLARY TANGLES AND SENILE PLAQUES

被引：35

作者：

GARVEY, W

FATHI, A

BIGELOW, F

JIMENEZ, CL

CARPENTER, BF

机构：

[1] Children’s Hospital of Eastern Ontario, Pathology Department, Ottawa, ON, KlH 8L1

来源：

JOURNAL OF HISTOTECHNOLOGY | 1991年 / 14卷 / 01期

关键词：

NEUROFIBRILLARY TANGLES; NERVE FIBERS; PHYSICAL DEVELOPER; SENILE PLAQUES; SILVER;

D O I：

10.1179/his.1991.14.1.39

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A simple, rapid, sensitive, consistent and economical method for plaques and tangles of Alzheimer's disease is presented. Sections were sensitized in 0.5% ammoniacal silver nitrate, followed by treatment in gum mastic and immersion in a physical developer consisting of hydroquinone, gum mastic and a very low concentration of silver nitrate. They were then treated with gold chloride and sodium thiosulfate. The procedure gives comparable results to those obtained with Bielchowsky and its modifications in about 30 minutes.

引用

页码：39 / 42

页数：4

共 19 条

[1]

Bancroft J.D., Stevens A., Theory and Practice of Histological Techniques, pp. 136-137, (1982)

[2]

Holmes W., Holmes method for nerve cells and fibers, Anta Red, 86, pp. 157-187, (1943)

[3]

Palmgren A., A rapid method for selective silver staining of nerve fibers and nerve endings in mounted paraffin sections, Actazoologica, 29, pp. 377-392, (1948)

[4]

Marsland T.A., Glees P., Erickson L.B., Modification of the Glees silver impregnation for paraffin sections, J Neuropath Exp Neurol, 13, (1954)

[5]

Hirano A., Zimmerman H.M., Hirano-Zimmerman method for nerve cells and fibers, Arch Neurol, 6, pp. 114-122, (1962)

[6]

Goodwin J.R., Chloral hydrate as a reducer in silver impregnation methods for the demonstration of axons and neurofibrils, Tech Bull Histotechnol, 15, pp. 132-231, (1985)

[7]

Gough N.G., The staining of nerve fibers in serial sections, J Anat, 106, pp. 437-448, (1970)

[8]

Bodian D., A new method for staining nerve fibers and nerve endings in mounted paraffin sections, Anat Rec, 65, pp. 89-97, (1936)

[9]

Loots J.M., Loots G.P., JoubertWS: A silver impregnation method for nervous tissue suitable for routine use with mounted sections, Stain Technol, 52, pp. 85-87, (1977)

[10]

Grimelius L., Olsson Y., A silver-gold impregnation technique for routine neuropathological use, Acta Neuropath (Berl), 41, pp. 161-164, (1978)

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