CLONING AND EXPRESSION OF A CHITINASE GENE FROM AEROMONAS-HYDROPHILA IN ESCHERICHIA-COLI

被引:29
作者
CHEN, JP
NAGAYAMA, F
CHANG, MC
机构
[1] DEPT FOOD & AGR, COUNCIL AGR, TAIPEI, TAIWAN
[2] TOKYO UNIV FISHERIES, DEPT FOOD SCI & TECHNOL, TOKYO 108, JAPAN
关键词
D O I
10.1128/AEM.57.8.2426-2428.1991
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
An extracellular secreted chitinase gene from Aeromonas hydrophila was cloned in Escherichia coli, and the gene product was detected in the culture medium. Like the natural chitinase protein, the excreted chitinase had a molecular weight of approximately 85,000 and was subject to catabolite repression by glucose.
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页码:2426 / 2428
页数:3
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