CLONING AND EXPRESSION OF THE CDNA CODING FOR THE ERYTHROCYTE ISOENZYME OF HUMAN ACYLPHOSPHATASE

被引：21

作者：

FIASCHI, T ^{[1
]}

RAUGEI, G ^{[1
]}

MARZOCCHINI, R ^{[1
]}

CHIARUGI, P ^{[1
]}

CIRRI, P ^{[1
]}

RAMPONI, G ^{[1
]}

机构：

[1] UNIV FLORENCE,DEPT BIOCHEM SCI,I-50134 FLORENCE,ITALY

来源：

FEBS LETTERS | 1995年 / 367卷 / 02期

关键词：

ACYLPHOSPHATASE; RECOMBINANT PROTEIN; CDNA; POLYMERASE CHAIN REACTION;

D O I：

10.1016/0014-5793(95)00553-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three independent cDNAs coding for the erythrocyte isoform of human acylphosphatase were isolated and characterized. Ah the clones were incomplete at the 5' end, but Northern blot analysis using the cDNA as a probe showed the presence of an unusually long mRNA 5'-untranslated region. The transcript was present in a variety of human cell lines of different origins, although at different levels, Southern blot analysis on DNA from different individuals revealed a simple hybridization pattern. Large amounts of pure enzyme with kinetic characteristics very similar to those of the native protein were expressed in E. coli.

引用

页码：145 / 148

页数：4

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