ANTIPROLIFERATIVE EFFECT OF PROSTAGLANDIN E(2) IN CULTURED GUINEA-PIG TRACHEAL SMOOTH-MUSCLE CELLS

被引:39
作者
FLORIO, C [1 ]
MARTIN, JG [1 ]
STYHLER, A [1 ]
HEISLER, S [1 ]
机构
[1] MCGILL UNIV,MONTREAL CHEST HOSP,RES CTR,MEAKINS CHRISTIE LABS,RES HLTH NETWORK CTR EXCELL,MONTREAL H2X 2P2,PQ,CANADA
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1994年 / 266卷 / 02期
关键词
AIRWAY SMOOTH MUSCLE CELLS; FORSKOLIN; ADENOSINE; 3'; 5'-CYCLIC MONOPHOSPHATE; GROWTH INHIBITION; INDOMETHACIN;
D O I
10.1152/ajplung.1994.266.2.L131
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
The respiratory epithelium plays an important role in modulating airway smooth muscle responsiveness to contractile agonists, and damage of the epithelium may be involved in the pathogenesis of bronchial hyperresponsiveness. This study was undertaken to determine whether prostaglandin E(2) (PGE(2)), a relaxant agent synthesized and released by airway epithelial cells, could exert long-term effects on airway smooth muscle by regulating cell proliferation. Incubation of growth arrested tracheal smooth muscle cells with serum for 24 h stimulated DNA synthesis in a concentration-dependent manner, as determined by [H-3]thymidine incorporation. PGE(2) and forskolin, a direct activator of adenylate cyclase, inhibited serum-induced cell proliferation, and the effects were dose dependent. PGE(2) and forskolin also stimulated adenosine 3',5'-cyclic monophosphate accumulation. An inhibitor of cyclooxygenase, indomethacin, enhanced DNA synthesis induced by serum. These results indicate that exogenous PGE(2) exerts an antiproliferative effect on smooth muscle cells in culture by activation of adenylate cyclase and suggest a role for the epithelium in modulating airway smooth muscle growth.
引用
收藏
页码:L131 / L137
页数:7
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