A MODIFICATION OF THE ELISA-DOUBLE SANDWICH METHOD FOR ESTIMATING THE CONCENTRATION OF HABUTOBIN

被引:6
作者
NAKAMURA, M [1 ]
HANASHIRO, K [1 ]
KOSUGI, T [1 ]
机构
[1] UNIV RYUKYUS,SCH MED,COMPREHENS MED RES CTR,NISHIHARA,OKINAWA 90301,JAPAN
关键词
D O I
10.1016/0041-0101(93)90405-8
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
A monoclonal antibody to the thrombin-like enzyme, habutobin, was produced. An ELISA-double sandwich method employing this monoclonal antibody was devised as a method for determining the habutobin concentration in vitro. However, the absorbance of the control sample in such an ELISA-double sandwich procedure was too high to estimate low levels of the enzyme. The present study therefore attempted to establish a reliable ELISA-double sandwich method in which the absorbance of the control sample was lower than previously, and which had a high sensitivity, in order to determine the habutobin concentration in vitro and in vivo. The modification of the ELISA-double sandwich technique employing the monoclonal antibody against habutobin, the purified rat IgG against habutobin and POD-mouse anti-rat IgG2a, provided a reliable means of determining the habutobin levels in the circulating blood of rabbits.
引用
收藏
页码:1325 / 1328
页数:4
相关论文
共 9 条
[1]  
KOSUGI T, 1986, THROMB HAEMOSTASIS, V55, P24
[2]  
LWIN KO, 1982, SNAKE, V14, P77
[3]   PRODUCTION OF A MONOCLONAL-ANTIBODY AGAINST THE THROMBIN-LIKE ENZYME, HABUTOBIN, FROM TRIMERESURUS-FLAVOVIRIDIS VENOM [J].
NAKAMURA, M ;
KINJOH, K ;
KOSUGI, T .
TOXICON, 1992, 30 (10) :1177-1188
[4]  
NAKAMURA M, 1992, J PHYSL, V446
[5]  
NOTERMANS S, 1982, METHOD ENZYMOL, V84, P223
[6]  
OUCHTERLONY O, 1953, ACTA PATHOL MIC SC, V32, P231
[7]  
THEAKSTON RDG, 1981, J TROP MED HYG, V84, P109
[8]  
THEAKSTON RDG, 1977, LANCET, V2, P639
[9]  
WATANABE M, 1988, Snake, V20, P25