QUANTIFICATION OF PROTEINS IN SAMPLE BUFFER FOR SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL-ELECTROPHORESIS USING COLLOIDAL SILVER

被引：35

作者：

DRABER, P

机构：

[1] Institute of Molecular Genetics, Czechoslovak Academy of Sciences

来源：

ELECTROPHORESIS | 1991年 / 12卷 / 06期

关键词：

D O I：

10.1002/elps.1150120617

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and simple assay for quantification of proteins in sodium dodecyl sulfate-sample buffer is described. Proteins are bound to a nitrocellulose membrane, stained with colloidal silver, and quantified by transmission densitometry. The staining requires 1-mu-L of protein sample and takes less than 20 min. Good linearity between the staining intensity and amount of proteins is in the range of 5-100 ng.

引用

页码：453 / 456

页数：4

共 13 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2]

DRABER P, 1989, J CELL SCI, V92, P519

[3]

FASMAN GD, 1976, HDB BIOCH MOL BIOL, V2, P383

[4] CAREY LEAS COLLOIDAL SILVER [J].