学术探索
学术期刊
新闻热点
数据分析
智能评审
立即登录
INCREASED PCR SENSITIVITY BY USING PARAFFIN WAX AS A REACTION MIX OVERLAY
被引:30
作者
:
HEBERT, B
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV QUEBEC,INST ARMAND FRAPPIER,CTR RECH IMMUNOL,531 BLVD PRAIRIES,LAVAL H7N 4Z3,QUEBEC,CANADA
HEBERT, B
BERGERON, J
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV QUEBEC,INST ARMAND FRAPPIER,CTR RECH IMMUNOL,531 BLVD PRAIRIES,LAVAL H7N 4Z3,QUEBEC,CANADA
BERGERON, J
POTWOROWSKI, EF
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV QUEBEC,INST ARMAND FRAPPIER,CTR RECH IMMUNOL,531 BLVD PRAIRIES,LAVAL H7N 4Z3,QUEBEC,CANADA
POTWOROWSKI, EF
TIJSSEN, P
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV QUEBEC,INST ARMAND FRAPPIER,CTR RECH IMMUNOL,531 BLVD PRAIRIES,LAVAL H7N 4Z3,QUEBEC,CANADA
TIJSSEN, P
机构
:
[1]
UNIV QUEBEC,INST ARMAND FRAPPIER,CTR RECH IMMUNOL,531 BLVD PRAIRIES,LAVAL H7N 4Z3,QUEBEC,CANADA
[2]
UNIV QUEBEC,INST ARMAND FRAPPIER,CTR RECH VIROL,LAVAL H7N 4Z3,QUEBEC,CANADA
来源
:
MOLECULAR AND CELLULAR PROBES
|
1993年
/ 7卷
/ 03期
关键词
:
PCR;
HOT START;
PARAFFIN WAX OVERLAY;
PCR SENSITIVITY;
D O I
:
10.1006/mcpr.1993.1036
中图分类号
:
Q5 [生物化学];
学科分类号
:
071010 ;
081704 ;
摘要
:
The use of mineral oil as a reaction mix overlay in conventional PCR may lead to problems. In addition to more difficult handling, traces of mineral oil in amplicon suspensions have been shown to decrease the efficiency of post-PCR manipulations. Commercial alternatives aimed at resolving the problem more than double the cost of an amplification. This is an important drawback when dealing with a large number of clinical samples. The use of inexpensive paraffin wax as a reaction mix overlay eliminated problems associated with the presence of mineral oil while being more practical and safer in handling potentially contaminated clinical samples. Moreover, when used in conjunction with a modified hot start technique, the use of paraffin wax increased the specificity and sensitivity of PCR amplifications over mineral oil in similar hot start conditions. Using the aforementioned method, the increase in specificity and sensitivity has enabled specific detection of viral DNA in clinical samples which the conventional PCR method failed to detect. © 1993 Academic Press, Limited.
引用
收藏
页码:249 / 252
页数:4
相关论文
共 8 条
[1]
BERGERON J, 1993, UNPUB PORCINE PARVOV, V1
[2]
BERGERON J, 1993, UNPUB PORCINE PARVOV, V2
[3]
BERGERON J, 1992, P INT PIG VET SOC, V1, P99
[4]
TYROSINE KINASE ACTIVATION IN THYMIC EPITHELIAL-CELLS - NECESSITY OF THYMOCYTE CONTACT THROUGH THE GP23/45/90 ADHESION COMPLEX
COUTURE, C
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV QUEBEC, INST ARMAND FRAPPIER, CTR RECH & IMMUNOL, POB 100, LAVAL H7N 4Z3, QUEBEC, CANADA
UNIV QUEBEC, INST ARMAND FRAPPIER, CTR RECH & IMMUNOL, POB 100, LAVAL H7N 4Z3, QUEBEC, CANADA
COUTURE, C
AMARANTEMENDES, G
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV QUEBEC, INST ARMAND FRAPPIER, CTR RECH & IMMUNOL, POB 100, LAVAL H7N 4Z3, QUEBEC, CANADA
UNIV QUEBEC, INST ARMAND FRAPPIER, CTR RECH & IMMUNOL, POB 100, LAVAL H7N 4Z3, QUEBEC, CANADA
AMARANTEMENDES, G
POTWOROWSKI, EF
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV QUEBEC, INST ARMAND FRAPPIER, CTR RECH & IMMUNOL, POB 100, LAVAL H7N 4Z3, QUEBEC, CANADA
UNIV QUEBEC, INST ARMAND FRAPPIER, CTR RECH & IMMUNOL, POB 100, LAVAL H7N 4Z3, QUEBEC, CANADA
POTWOROWSKI, EF
[J].
EUROPEAN JOURNAL OF IMMUNOLOGY,
1992,
22
(10)
: 2579
-
2585
[5]
MAXIMIZING SENSITIVITY AND SPECIFICITY OF PCR BY PREAMPLIFICATION HEATING
DAQUILA, RT
论文数:
0
引用数:
0
h-index:
0
机构:
HARVARD UNIV,SCH MED,BOSTON,MA 02129
HARVARD UNIV,SCH MED,BOSTON,MA 02129
DAQUILA, RT
BECHTEL, LJ
论文数:
0
引用数:
0
h-index:
0
机构:
HARVARD UNIV,SCH MED,BOSTON,MA 02129
HARVARD UNIV,SCH MED,BOSTON,MA 02129
BECHTEL, LJ
VIDELER, JA
论文数:
0
引用数:
0
h-index:
0
机构:
HARVARD UNIV,SCH MED,BOSTON,MA 02129
HARVARD UNIV,SCH MED,BOSTON,MA 02129
VIDELER, JA
ERON, JJ
论文数:
0
引用数:
0
h-index:
0
机构:
HARVARD UNIV,SCH MED,BOSTON,MA 02129
HARVARD UNIV,SCH MED,BOSTON,MA 02129
ERON, JJ
GORCZYCA, P
论文数:
0
引用数:
0
h-index:
0
机构:
HARVARD UNIV,SCH MED,BOSTON,MA 02129
HARVARD UNIV,SCH MED,BOSTON,MA 02129
GORCZYCA, P
KAPLAN, JC
论文数:
0
引用数:
0
h-index:
0
机构:
HARVARD UNIV,SCH MED,BOSTON,MA 02129
HARVARD UNIV,SCH MED,BOSTON,MA 02129
KAPLAN, JC
[J].
NUCLEIC ACIDS RESEARCH,
1991,
19
(13)
: 3749
-
3749
[6]
SELECTIVE EXTRACTION OF POLYOMA DNA FROM INFECTED MOUSE CELL CULTURES
HIRT, B
论文数:
0
引用数:
0
h-index:
0
HIRT, B
[J].
JOURNAL OF MOLECULAR BIOLOGY,
1967,
26
(02)
: 365
-
&
[7]
Mullis K B, 1991, PCR Methods Appl, V1, P1
[8]
TIJSSEN P, 1993, HYBRIDIZATION NUCLEI
←
1
→
共 8 条
[1]
BERGERON J, 1993, UNPUB PORCINE PARVOV, V1
[2]
BERGERON J, 1993, UNPUB PORCINE PARVOV, V2
[3]
BERGERON J, 1992, P INT PIG VET SOC, V1, P99
[4]
TYROSINE KINASE ACTIVATION IN THYMIC EPITHELIAL-CELLS - NECESSITY OF THYMOCYTE CONTACT THROUGH THE GP23/45/90 ADHESION COMPLEX
COUTURE, C
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV QUEBEC, INST ARMAND FRAPPIER, CTR RECH & IMMUNOL, POB 100, LAVAL H7N 4Z3, QUEBEC, CANADA
UNIV QUEBEC, INST ARMAND FRAPPIER, CTR RECH & IMMUNOL, POB 100, LAVAL H7N 4Z3, QUEBEC, CANADA
COUTURE, C
AMARANTEMENDES, G
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV QUEBEC, INST ARMAND FRAPPIER, CTR RECH & IMMUNOL, POB 100, LAVAL H7N 4Z3, QUEBEC, CANADA
UNIV QUEBEC, INST ARMAND FRAPPIER, CTR RECH & IMMUNOL, POB 100, LAVAL H7N 4Z3, QUEBEC, CANADA
AMARANTEMENDES, G
POTWOROWSKI, EF
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV QUEBEC, INST ARMAND FRAPPIER, CTR RECH & IMMUNOL, POB 100, LAVAL H7N 4Z3, QUEBEC, CANADA
UNIV QUEBEC, INST ARMAND FRAPPIER, CTR RECH & IMMUNOL, POB 100, LAVAL H7N 4Z3, QUEBEC, CANADA
POTWOROWSKI, EF
[J].
EUROPEAN JOURNAL OF IMMUNOLOGY,
1992,
22
(10)
: 2579
-
2585
[5]
MAXIMIZING SENSITIVITY AND SPECIFICITY OF PCR BY PREAMPLIFICATION HEATING
DAQUILA, RT
论文数:
0
引用数:
0
h-index:
0
机构:
HARVARD UNIV,SCH MED,BOSTON,MA 02129
HARVARD UNIV,SCH MED,BOSTON,MA 02129
DAQUILA, RT
BECHTEL, LJ
论文数:
0
引用数:
0
h-index:
0
机构:
HARVARD UNIV,SCH MED,BOSTON,MA 02129
HARVARD UNIV,SCH MED,BOSTON,MA 02129
BECHTEL, LJ
VIDELER, JA
论文数:
0
引用数:
0
h-index:
0
机构:
HARVARD UNIV,SCH MED,BOSTON,MA 02129
HARVARD UNIV,SCH MED,BOSTON,MA 02129
VIDELER, JA
ERON, JJ
论文数:
0
引用数:
0
h-index:
0
机构:
HARVARD UNIV,SCH MED,BOSTON,MA 02129
HARVARD UNIV,SCH MED,BOSTON,MA 02129
ERON, JJ
GORCZYCA, P
论文数:
0
引用数:
0
h-index:
0
机构:
HARVARD UNIV,SCH MED,BOSTON,MA 02129
HARVARD UNIV,SCH MED,BOSTON,MA 02129
GORCZYCA, P
KAPLAN, JC
论文数:
0
引用数:
0
h-index:
0
机构:
HARVARD UNIV,SCH MED,BOSTON,MA 02129
HARVARD UNIV,SCH MED,BOSTON,MA 02129
KAPLAN, JC
[J].
NUCLEIC ACIDS RESEARCH,
1991,
19
(13)
: 3749
-
3749
[6]
SELECTIVE EXTRACTION OF POLYOMA DNA FROM INFECTED MOUSE CELL CULTURES
HIRT, B
论文数:
0
引用数:
0
h-index:
0
HIRT, B
[J].
JOURNAL OF MOLECULAR BIOLOGY,
1967,
26
(02)
: 365
-
&
[7]
Mullis K B, 1991, PCR Methods Appl, V1, P1
[8]
TIJSSEN P, 1993, HYBRIDIZATION NUCLEI
←
1
→