NUCLEOTIDE-SEQUENCE OF LEVANSUCRASE GENE (LEVU) OF ZYMOMONAS-MOBILIS ZM1 (ATCC10988)

被引：48

作者：

SONG, KB

JOO, HK

RHEE, SK

机构：

[1] KIST,GERI,METABOL ENGN LAB,POB 17,TAEJON 305606,SOUTH KOREA

[2] KONKUK UNIV,DEPT AGR CHEM,SEOUL,SOUTH KOREA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1173卷 / 03期

关键词：

LEVANSUCRASE GENE; DNA SEQUENCE; (Z-MOBILIS);

D O I：

10.1016/0167-4781(93)90130-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The extracellular levansucrase gene (levU) was cloned from the genomic DNA of Zymomonas mobilis ZM1 and the nucleotide sequence of the levU structural gene was determined. The 3.1 kb EcoRV-polylinker fusion DNA fragment containing the levU gene had an open reading frame of 1272 bps and the deduced amino acid sequence was 423 residues long with a molecular weight of 46725. Although this protein exhibited little similarity with other known levansucrases, several well-conserved regions were observed (1-5 regions).

引用

页码：320 / 324

页数：5

共 20 条

[1]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[2] NUCLEOTIDE-SEQUENCE OF THE SUCRASE GENE OF BACILLUS-SUBTILIS [J].