RADIOIMMUNOASSAY OF CYTIDINE 3',5'-CYCLIC-MONOPHOSPHATE - UNAMBIGUOUS ASSAY BY MEANS OF AN OPTIMIZED PROTOCOL INCORPORATING A TRILAYER COLUMN SEPARATION TO OBVIATE CROSS-REACTIVITY PROBLEMS

被引：7

作者：

NEWTON, RP

EVANS, AM

VANGEYSCHEM, J

DIFFLEY, PJ

HASSAM, HG

HAKEEM, NA

MOYSE, CD

COOKE, R

SALVAGE, BJ

机构：

[1] Biochemistry Group, School of Biological Sciences, University College Swansea, Swansea

来源：

JOURNAL OF IMMUNOASSAY | 1994年 / 15卷 / 04期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1080/15321819408009581

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous assays for cytidine 3',5'-cyclic monophosphate (cyclic CMP) have been criticized as being ambiguous. Here a modified RIA protocol, in which the production of assay components has been optimized and a novel trilayer chromatography column separation introduced which successfully separates cyclic CMP from compounds, endogenous to living tissues, which cross-react with anti-cyclic CMP sera, is described. The assay is capable of assaying cyclic CMP between 0.1 and 5 pmol, can be increased in sensitivity by means of an additional acetylation step, and enables the separation of cyclic CMP, cyclic AMP and cyclic GMP so that all three can be estimated in a single sample.

引用

页码：317 / 337

页数：21

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