CONSTRUCTION OF A YAC CONTIG AND A STS MAP SPANNING AT LEAST 7 MEGABASEPAIRS IN CHROMOSOME-5Q34-35

被引：18

作者：

LUKUO, JM

LEPASLIER, D

WEISSENBACH, J

CHUMAKOV, I

COHEN, D

WARD, DC

机构：

[1] YALE UNIV,SCH MED,DEPT MOLEC BIOPHYS & BIOCHEM,NEW HAVEN,CT 06510

[2] YALE UNIV,SCH MED,DEPT GENET,NEW HAVEN,CT 06510

[3] CTR ETUD POLYMORPHISME HUMAIN,F-75010 PARIS,FRANCE

[4] GENETHON,F-9100 EVRY,FRANCE

[5] INST PASTEUR,F-75724 PARIS,FRANCE

来源：

HUMAN MOLECULAR GENETICS | 1994年 / 3卷 / 01期

关键词：

D O I：

10.1093/hmg/3.1.99

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have constructed a YAC contig containing 54 clones and a minimum of 7 Mbp of human DNA, that maps to bands q34 - 35 on chromosome 5. The contig was nucleated using FISH mapped cosmid clones shown to flank the t(2;5)(p23;q35) translocation breakpoint in a CD30-positive large cell lymphoma cell line. Thirty of the 54 YAC clones are non-chimeric and six span the translocation breakpoint, as determined by FISH analysis. A total of 28 YAC clone end fragments, 14 non-polymorphic YAC end STS probes and 13 polymorphic microsatellite STS markers have been used to order clones within the contig. The most distal genetic markers (D5S498 and D5S619) are separated by 15 cM based on multipoint linkage analysis. This map of overlapping clones and the set of densely spaced physical markers will promote our understanding of the 5q34 - 35 region and its associated genes.

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页码：99 / 106

页数：8

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