ACTIVATION OF TSK AND BTK TYROSINE KINASES BY G-PROTEIN BETA-GAMMA-SUBUNITS

被引：124

作者：

LANGHANSRAJASEKARAN, SA ^{[1
]}

WAN, Y ^{[1
]}

HUANG, XY ^{[1
]}

机构：

[1] CORNELL UNIV,COLL MED,DEPT PHYSIOL,NEW YORK,NY 10021

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 19期

关键词：

PLECKSTRIN-HOMOLOGY DOMAIN; SIGNAL TRANSDUCTION;

D O I：

10.1073/pnas.92.19.8601

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Tsk/Itk and Btk are members of the pleckstrin-homology (PH) domain-containing tyrosine kinase family. The PH domain has been demonstrated to be able to interact with beta gamma subunits of heterotrimeric guanine nucleotide-binding proteins (G proteins) (G(beta gamma)) and phospholipids. Using cotransfection assays, we show here that the kinase activities of Tsk and Btk are stimulated by certain G(beta gamma) subunits. Furthermore, using an in vitro reconstitution assay with purified bovine brain G(beta gamma) subunits and the immunoprecipitated Tsk, we find that Tsk kinase activity is increased by G(beta gamma) subunits and another membrane factor(s). These results indicate that this family of tyrosine kinases could be an effector of heterotrimeric G proteins.

引用

页码：8601 / 8605

页数：5

共 41 条

[1] G-ALPHA-16, A G-PROTEIN ALPHA SUBUNIT SPECIFICALLY EXPRESSED IN HEMATOPOIETIC-CELLS [J].

AMATRUDA, TT ;

STEELE, DA ;

SLEPAK, VZ ;

SIMON, MI .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (13) :5587-5591

[2] CD28 IS ASSOCIATED WITH AND INDUCES THE IMMEDIATE TYROSINE PHOSPHORYLATION AND ACTIVATION OF THE TEC FAMILY KINASE ITK/EMT IN THE HUMAN JURKAT LEUKEMIC T-CELL LINE [J].

AUGUST, A ;

GIBSON, S ;

KAWAKAMI, Y ;

KAWAKAMI, T ;

MILLS, GB ;

DUPONT, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (20) :9347-9351

[3] BINDING OF BRUTONS TYROSINE KINASE TO FYN, LYN, OR HCK THROUGH A SRC HOMOLOGY-3 DOMAIN-MEDIATED INTERACTION [J].

CHENG, GH ;

YE, ZS ;

BALTIMORE, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (17) :8152-8155

[4]

CIFUENTES ME, 1993, J BIOL CHEM, V268, P11586

[5] NEW ROLES FOR G-PROTEIN BETA-GAMMA-DIMERS IN TRANSMEMBRANE SIGNALING [J].

CLAPHAM, DE ;

NEER, EJ .

NATURE, 1993, 365 (6445) :403-406

[6] RAS-DEPENDENT ACTIVATION OF MAP KINASE PATHWAY MEDIATED BY G-PROTEIN BETA-GAMMA-SUBUNITS [J].

CRESPO, P ;

XU, NZ ;

SIMONDS, WF ;

GUTKIND, JS .

NATURE, 1994, 369 (6479) :418-420

[7]

DAVIS LH, 1994, J BIOL CHEM, V269, P4409

[8]

FAURE M, 1994, J BIOL CHEM, V269, P7851

[9] CRYSTAL-STRUCTURE AT 2.2-ANGSTROM RESOLUTION OF THE PLECKSTRIN HOMOLOGY DOMAIN FROM HUMAN DYNAMIN [J].

FERGUSON, KM ;

LEMMON, MA ;

SCHLESSINGER, J ;

SIGLER, PB .

CELL, 1994, 79 (02) :199-209

[10]

GILMAN AG, 1987, ANNU REV BIOCHEM, V56, P615, DOI 10.1146/annurev.bi.56.070187.003151

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