PURIFICATION OF MEROZOITES OF THEILERIA-SERGENTI FROM INFECTED BOVINE ERYTHROCYTES

被引：41

作者：

SUGIMOTO, C ^{[1
]}

SATO, M ^{[1
]}

KAWAZU, S ^{[1
]}

KAMIO, T ^{[1
]}

FUJISAKI, K ^{[1
]}

机构：

[1] NATL INST ANIM HLTH,TSUKUBA,IBARAKI 305,JAPAN

来源：

PARASITOLOGY RESEARCH | 1991年 / 77卷 / 02期

关键词：

D O I：

10.1007/BF00935426

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A simple and rapid method for purifying merozoites of Theileria sergenti from infected bovine erythrocytes was developed. Infected erythrocytes were lysed by the use of a cytolytic toxin produced by Aeromonas hydrophila and the lysate was subjected to ultracentrifugation in a Percoll discontinuous density gradient. Pure and morphologically intact merozoites free of erythrocyte membrane were recovered from a band formed at the interface of 40% and 60% (by vol.) Percoll solutions. In this merozoite fraction, contamination of erythrocyte membrane proteins was not detected as examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

引用

页码：129 / 131

页数：3

共 11 条

[1] PURIFICATION AND SOME PROPERTIES OF AEROMONAS-HYDROPHILA HEMOLYSIN [J].