SIMULTANEOUS DETERMINATION OF TESTOSTERONE AND ANDROSTADIENONE (SEX ATTRACTANT) IN HUMAN PLASMA BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY WITH HIGH-RESOLUTION SELECTED-ION MONITORING

被引：10

作者：

FUKUSHIMA, S ^{[1
]}

AKANE, A ^{[1
]}

MATSUBARA, K ^{[1
]}

SHIONO, H ^{[1
]}

MORISHITA, H ^{[1
]}

NAKADA, F ^{[1
]}

机构：

[1] SHIMANE MED UNIV,DEPT CHEM,IZUMO,SHIMANE 693,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 565卷 / 1-2期

关键词：

D O I：

10.1016/0378-4347(91)80368-M

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Androsta-4, 16-dien-3-one (androstadienone) and androst-4-en-3-one-17-beta-ol (testosterone) in healthy human plasma were simultaneously determined under several experimental conditions by gas chromatography-mass spectrometry with high-resolution selected-ion monitoring. Internal standards were [2,2,4,6,6-(H5)-H-2]androstadienone and [2,2,4,6,6,-(H5)-H-2]testosterone. Samples were extracted with an Extrelut(R) column, purified using Lipidex(R) 5000 and converted into hydroxime-trimethylsilyl derivatives for determination. Physiological concentrations of androstadienone and testosterone found in eleven healthy men were 2.05 +/- 0.74 and 18.6 +/- 4.9 pmol/ml in plasma (mean +/- S.D.), respectively. No correlation was observed between these steroid concentrations.

引用

页码：35 / 44

页数：10

共 18 条

[1] BIOSYNTHESIS OF SOME ANDROST-16-ENES FROM C21 AND CU9 STEROIDS IN BOAR TESTICULAR AND ADRENAL TISSUE [J].