KINETOPLAST-ASSOCIATED DNA TOPOISOMERASE IN CRITHIDIA-FASCICULATA - CROSS-LINKING OF MITOCHONDRIAL TOPOISOMERASE-II TO BOTH MINICIRCLES AND MAXICIRCLES IN CELLS TREATED WITH THE TOPOISOMERASE INHIBITOR VP16

被引：18

作者：

RAY, DS ^{[1
]}

HINES, JC ^{[1
]}

ANDERSON, M ^{[1
]}

机构：

[1] UNIV CALIF LOS ANGELES,DEPT BIOL,LOS ANGELES,CA 90024

来源：

NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 13期

关键词：

D O I：

10.1093/nar/20.13.3353

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mitochondrial DNA of the trypanosomatid Crithidia fasciculata consists of thousands of copies of a 2.5 kb minicircle and a small number of 37kb maxicircles catenated into a single enormous network. Treatment of C. fasciculata with the type II DNA topoisomerase inhibitor VP16 produces cleavable complexes of a type II DNA topoisomerase with both minicircles and maxicircles. A combined Southern and Western blot analysis of the cleaved DNA species released from the network by SDS treatment has identified topollmt, the kinetoplast-associated topoisomerase, in covalent complexes with linear forms of minicircle and maxicircle DNAs. These results directly implicate topollmt in the topological reactions required for the duplication of the kinetoplast network.

引用

页码：3353 / 3356

页数：4

共 27 条

[1] A MODEL FOR RNA EDITING IN KINETOPLASTID MITOCHONDRIA - GUIDE RNA MOLECULES TRANSCRIBED FROM MAXICIRCLE DNA PROVIDE THE EDITED INFORMATION [J].