CRYSTALS OF BETA-XYLANASE FROM ASPERGILLUS-ORYZAE

被引：5

作者：

GOLUBEV, AM ^{[1
]}

KILIMNIK, AY ^{[1
]}

NEUSTROEV, KN ^{[1
]}

PICKERSGILL, RW ^{[1
]}

机构：

[1] INST FOOD RES, READING LAB, READING RG6 2EF, ENGLAND

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1993年 / 230卷 / 02期

关键词：

XYLANASE; CRYSTALS; FUNGUS;

D O I：

10.1006/jmbi.1993.1177

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An endo-xylanase was isolated from the culture of fungus Aspergillus oryzae variant D5. The purified enzyme had a molecular weight of 24,000 and the isoelectric point of 3.6. Xylanase crystals were obtained from a polyethylene glycol 6000 solution by the hanging-drop method. Seeding was used for the enlargement of the crystal size. Crystals belong to the monoclinic space group P21 with cell dimensions a = 54.9 Å, b = 74.5 Å, c = 50.8 Å, and β = 108.7°. Crystals diffract beyond 2.5 Å resolution. © 1993 Academic Press, Inc.

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页码：661 / 663

页数：3

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