HEMATOXYLIN STAINING OF TISSUES EMBEDDED IN EPOXY RESINS

被引：21

作者：

SHIRES, TK

JOHNSON, M

RICHTER, KM

机构：

[1] Departments of Anatomy and Urology, University of Oklahoma School of Medicine Cancer Section, Oklahoma Medical Research Foundation and Veterans Hospital, Oklahoma City, OK

来源：

STAIN TECHNOLOGY | 1969年 / 44卷 / 01期

关键词：

D O I：

10.3109/10520296909063319

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Sections of 0.5-2 μ thickness are affixed to slides with albumen adhesive, thoroughly dried, and placed in xylene or toluene for 1 hr, then brought through ethanol to water. Sections of tissue fixed in OsO4 are treated first in 0.1% KMnO4, then with 1.0% oxalic acid, and after rinsing, incubated at 60 C for 12-24 hr in hematoxylin (Harris's or Ehrlich's) and counterstained 10-15 min with 0.5% phloxine B. Permanent preparations are made by clearing and mounting in a synthetic resin. The method requires only easily available reagents and is suitable for routine processing of epoxy sections. © 1969 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：21 / +

页数：1

共 20 条

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