OVEREXPRESSION OF A LEADERLESS FORM OF YEAST CYTOCHROME-C-OXIDASE SUBUNIT-VA CIRCUMVENTS THE REQUIREMENT FOR A LEADER PEPTIDE IN MITOCHONDRIAL IMPORT

被引：14

作者：

DIRCKS, LK ^{[1
]}

POYTON, RO ^{[1
]}

机构：

[1] UNIV COLORADO,DEPT MOLEC CELLULAR & DEV BIOL,CAMPUS BOX 347,BOULDER,CO 80309

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1990年 / 10卷 / 09期

关键词：

D O I：

10.1128/MCB.10.9.4984

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Subunit Va of Saccharomyces cerevisiae cytochrome c oxidase is a nucleus-encoded mitochondrial protein that is derived from a precursor with a 20-residue leader peptide. We previously reported that this leader peptide is required for import of subunit Va into mitochondria in vivo (S. M. Glaser, C. E. Trueblood, L. K. Dircks, R. O. Poyton, and M. G. Cumsky, J. Cell. Biochem. 36:275-278, 1988). Here we show that overproduction of a leaderless form of subunit Va circumvents the leader peptide requirement for import into mitochondria in vivo.

引用

页码：4984 / 4986

页数：3

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