INTERACTION OF BOVINE ESTROGEN-RECEPTOR WITH IMMOBILIZED ZINC

被引：2

作者：

ATKINSON, HC ^{[1
]}

RATAJCZAK, T ^{[1
]}

HAHNEL, R ^{[1
]}

机构：

[1] UNIV WESTERN AUSTRALIA,KING EDWARD MEM HOSP WOMEN,DEPT OBSTET & GYNAECOL,ENDOCRINE RES LAB,SUBIACO,WA,AUSTRALIA

来源：

STEROIDS | 1990年 / 55卷 / 09期

关键词：

STEROIDS; ESTROGEN RECEPTOR; ZINC BINDING; SALT-DISSOCIATED RECEPTOR; DITHIOTHREITOL REQUIREMENT;

D O I：

10.1016/0039-128X(90)90099-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The metal-binding properties of partially purified untransformed or salt-dissociated bovine estrogen receptors were studied using zinc-chelated iminodiacetic acid gels. Only the salt-dissociated 5S recept or retained by the metal-chelated resin, and this interaction is dependent on the presence of dithiothreitol. The untransformed 9S receptor is not retained, indicating that the zinc-interacting amino acid residues may be masked by receptor-associated proteins such as 90K heat-shock protein or because of an unfavorable receptor conformation.

引用

页码：405 / 409

页数：5

共 26 条

[1]

Yamamoto, Steroid receptor regulated transcription of specific genes and gene networks, Annu Rev Genet, 19, pp. 209-252, (1985)

[2]

Skafar, Notides, Modulation of the estrogen receptor's affinity for DNA by estradiol, J Biol Chem, 260, pp. 12208-12213, (1985)

[3]

Sabbah, Redeuilh, Secco, Baulieu, The binding activity of estrogen receptor to DNA and heat shock protein (Mr 90,000) is dependent on receptor-bound metal, J BiolCkem, 20, pp. 8631-8635, (1987)

[4]

Hollenberg, Weinberger, Ong, Cerelli, Oro, Lebo, Thompson, Rosenfeld, Evans, Primary structure and expression of a functional human glucocorticoid receptor, Nature, 318, pp. 635-641, (1985)

[5]

Green, Walter, Kumar, Krust, Bomert, Argos, Chambon, Human estrogen receptor cDNA: sequence, expression and homology to v-erb-A, Nature, 320, pp. 134-139, (1986)

[6]

Jeltsch, Krozowski, Quirin-Stricker, Gronemeyer, Simpson, Gamier, Krust, Jacob, Chambon, Cloning of the chicken progesterone receptor, Proc Natl Acad Sci USA, 83, pp. 5424-5428, (1986)

[7]

Green, Chambon, A superfamily of potentially oncogenic hormone receptors, Nature, 324, pp. 615-617, (1986)

[8]

Chang, Kokontis, Identification of a new member of the steroid receptor superfamily by cloning and sequence analysis, Biochem Biophys Res Commun, 155, pp. 971-977, (1988)

[9]

Freedman, Luisi, Korszun, Basavappa, Sigler, Yamamoto, The function and structure of the metal coordination sites within the glucocorticoid receptor DNA binding domain, Nature, 334, pp. 543-546, (1988)

[10]

Porath, Carlsson, Olsson, Beifrage, Metal chelate affinity chromatography, a new approach to protein fractionation, Nature, 258, pp. 598-599, (1975)

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