ALTERNATIVE INITIATION OF TRANSLATION DETERMINES CYTOPLASMIC OR NUCLEAR-LOCALIZATION OF BASIC FIBROBLAST GROWTH-FACTOR

被引：324

作者：

BUGLER, B

AMALRIC, F

PRATS, H

机构：

[1] CNRS,CTR RECH BIOCHIM & GENET CELLULAIRES,118 ROUTE NARBONNE,F-31062 TOULOUSE,FRANCE

[2] CHU RANGUEIL,ENDOCRINOL EXPTL LAB,F-31054 TOULOUSE,FRANCE

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1991年 / 11卷 / 01期

关键词：

D O I：

10.1128/MCB.11.1.573

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three forms of basic fibroblast growth factor (bFGF), initiated at an AUG (18 kDa) and two CUG (21 and 22.5 kDa) start codons, were produced following transfection of COS cells with human hepatoma bFGF cDNA. The subcellular localization of the different forms was investigated directly or by using chimeric genes constructed by fusion of the bFGF and chloramphenicol acetyltransferase open reading frames. The AUG-initiated proteins were cytoplasmic, while the CUG-initiated forms were nuclear. The signal sequence responsible for the nuclear localization of bFGF is contained within 37 amino acid residues between the second CUG and the AUG start codons. Alternative initiation of translation regulates the subcellular localization of bFGF and thus could modulate its role in cell growth and differentiation control.

引用

页码：573 / 577

页数：5

共 14 条

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