CHEMICAL MODIFICATION OF ARGINYL RESIDUES IN SILK FIBROIN .1. REACTION OF 1,2-CYCLOHEXANEDIONE IN BORATE BUFFER

被引：10

作者：

GOTOH, Y ^{[1
]}

TSUKADA, M ^{[1
]}

MINOURA, N ^{[1
]}

机构：

[1] IND PROD RES INST,TSUKUBA,IBARAKI 305,JAPAN

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 1992年 / 14卷 / 04期

关键词：

SILK FIBROIN; CHEMICAL MODIFICATION; ARGINYL RESIDUE; 1,2-CYCLOHEXANEDIONE; BORATE; MEMBRANE;

D O I：

10.1016/S0141-8130(05)80027-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chemical modifications of silk fibroin were attempted in order to add new properties and functions to silk fibroin. The arginyl residue in solubilized silk fibroin was chemically modified with the reaction of 1,2-cyclohexanedione in borate buffer. FT-i.r. and c.d. spectra of the silk fibroin before and after the modification indicated that the fraction of random coil conformation increased with the modification. The chemical stability of the modified silk fibroin membrane was investigated in vitro with phosphate buffer. The modified arginyl residue in the membrane was considerably regenerated with the treatment in phosphate buffer.

引用

页码：198 / 200

页数：3

共 14 条

[1] PREPARATION AND PROPERTIES OF COVALENTLY IMMOBILIZED ALKALINE-PHOSPHATASE ON BOMBYX-MORI SILK FIBROIN FIBER [J].