RFLP ANALYSIS OF MYCOBACTERIUM MALMOENSE STRAINS USING RIBOSOMAL-RNA GENE PROBES - AN ADDITIONAL TOOL TO EXAMINE INTRASPECIES VARIATION

被引：13

作者：

KAUPPINEN, J

PELKONEN, J

KATILA, ML

机构：

[1] KUOPIO UNIV HOSP,DEPT CLIN MICROBIOL,SF-70211 KUOPIO,FINLAND

[2] UNIV KUOPIO,DEPT CLIN MICROBIOL,SF-70211 KUOPIO,FINLAND

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1994年 / 19卷 / 04期

关键词：

EPIDEMIOLOGY; MYCOBACTERIUM MALMOENSE; RFLP; RIBOSOMAL-RNA GENE;

D O I：

10.1016/0167-7012(94)90029-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

To examine the intraspecies variation of Mycobacterium malmoense, 29 clinical isolates, chemotypes by thin-layer chromatography of their surface glycolipids into five subgroups, were typed using RFLP analysis. Two (32)p-labelled ribosomal RNA (rRNA) gene fragments were used as probes for chromosomal DNA digested with BanI. By comparison of the fingerprint patterns, the strains could be divided into five ribotypes, two major and three minor ones. The chemotypes were not connected to any of the ribotypes. Combination of the results of the two techniques produced a considerable resolution. This method is a useful additional tool when studying the epidemiological behaviour of M. malmoense.

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页码：261 / 267

页数：7

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