A HOUSEKEEPING TYPE PROMOTER, LOCATED IN THE 3' REGION OF THE DUCHENNE MUSCULAR-DYSTROPHY GENE, CONTROLS THE EXPRESSION OF DP71, A MAJOR PRODUCT OF THE GENE

被引：67

作者：

LEDERFEIN, D ^{[1
]}

YAFFE, D ^{[1
]}

NUDEL, U ^{[1
]}

机构：

[1] WEIZMANN INST SCI, DEPT CELL BIOL, IL-76100 REHOVOT, ISRAEL

来源：

HUMAN MOLECULAR GENETICS | 1993年 / 2卷 / 11期

关键词：

D O I：

10.1093/hmg/2.11.1883

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The 70.8 kDa protein product of the distal part of the giant Duchenne muscular dystrophy (DMD) gene, Dp71, is expressed in many cell types and tissues. Anchored PCR, primer extension and functional analysis of transfected constructs were used to determine the 5' end of the mRNA and characterize the promoter of this major DMD gene product. The 5' untranslated region (5'UTR) of Dp71 is transcribed from a single exon; the promoter does not contain a TATA box, and has a very high GC content and several potential Sp1 binding sites. It is located more than 2000 kb 3' to the muscle and brain type dystrophin promoters and only 150 kb from the 3' end of the gene, suggesting that in most DMD patients the expression of Dp71 is unaffected.

引用

页码：1883 / 1888

页数：6

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