An Improved High Performance Liquid Chromatographic Post-column Derivatization Procedure for the UV-Vis Spectroscopic Characterization of Phenolic Compounds

被引：5

作者：

Mueller-Harvey, I. ^{[1
]}

Blackwell, P. M. S. ^{[1
]}

机构：

[1] AFRC Inst Grassland & Environm Res, Maidenhead SL6 5LR, Berks, England

来源：

PHYTOCHEMICAL ANALYSIS | 1991年 / 2卷 / 01期

关键词：

High performance liquid chromatography; post-column derivatization; phenolic compounds; UV-Vis spectroscopy;

D O I：

10.1002/pca.2800020108

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An improved method is described for the characterization of phenolic compounds using post-column shift reagents following their separation by high performance liquid chromatography (HPLC). Modifications include the use of NaOH to adjust the pH of the column effluent, prior to introducing the AlCl3 and H3BO3 reagents, and the use of commercially available spin chambers for efficiently mixing the effluent with the reagents, resulting in a negligible loss of resolution. Shifts obtained by the HPLC post-column derivatization and the conventional procedure are comparable.

引用

页码：38 / 42

页数：5

共 6 条

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