A NEW PROCEDURE FOR THE REDUCTION OF URONIC-ACID CONTAINING POLYSACCHARIDES

被引：16

作者：

FONTAINE, T

FOURNET, B

KARAMANOS, Y

机构：

[1] UNIV SCI & TECH LILLE FLANDRES ARTOIS, CNRS, UNITE MIXTE RECH 111, F-59655 VILLENEUVE DASCQ, FRANCE

[2] INST BIOTECHNOL, F-87060 LIMOGES, FRANCE

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1994年 / 20卷 / 02期

关键词：

DIAZOMETHANE; METHYLESTERIFICATION; POLYSACCHARIDE; REDUCTION; URONIC ACID;

D O I：

10.1016/0167-7012(94)90017-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The presence of uronic acid residues in polysaccharide induces a difficult quantitative determination of monosaccharides. Reduction of uronic acid into corresponding hexose allows a complete monosaccharide liberation by acidic treatment. However, reduction methods previously described have not permitted a quantitative reaction. This paper describes a new quantitative reduction procedure for the uronic acid containing different polysaccharides. Uronic acid reduction is performed by methylesterification in dimethylsulfoxide/ methanol with diazomethane followed by reduction in imidazol buffer with potassium borohydride. Moreover, the technique allows a quantitative reaction of low quantities of polymer without polymer degradation.

引用

页码：149 / 157

页数：9

共 26 条

[1] A RADIOCHEMICAL APPROACH TO THE DETERMINATION OF CARBOXYLIC-ACID GROUPS IN POLYSACCHARIDES [J].