MOLECULAR CHARACTERIZATION OF HUMAN MONOCLONAL-ANTIBODIES SPECIFIC FOR SEVERAL HIV PROTEINS - ANALYSIS OF THE V(H)3 FAMILY EXPRESSION

被引：13

作者：

DAVID, D

GOOSSENS, D

DESGRANGES, C

THEZE, J

ZOUALI, M

机构：

[1] INST PASTEUR, UNITE IMMUNOGENET CELLULAIRE, F-75724 PARIS 15, FRANCE

[2] INTS, PARIS, FRANCE

[3] INSERM, U271, LYON, FRANCE

来源：

IMMUNOLOGY LETTERS | 1995年 / 47卷 / 1-2期

关键词：

HUMAN V-H GENE; ANTI-HIV ANTIBODY; B-CELL REPERTOIRE;

D O I：

10.1016/0165-2478(95)00078-J

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We have analyzed the heavy-chain variable (V-H) region genes expressed by a panel of human monoclonal antibodies derived from an immunized volunteer, an AIDS patient and seropositive asymptomatic donors, and specific for HIV-1 enu, pol and gag gene products. The third complementarity-determining regions show a high complexity with unconventional gene recombination events. Most of the V-H genes utilized are also frequently encountered in other immune responses. Their sequences are, in general, typical of an antigen-driven immune response. Molecular mechanisms that generate high-affinity antibodies are then effective during HIV infection. Remarkably, V(H)3 family, which dominates the human antibody repertoire, is barely encountered among anti-HIV antibodies.

引用

页码：107 / 112

页数：6

共 30 条

[1] RESTRICTED IMMUNOGLOBULIN VH USAGE AND VDJ COMBINATIONS IN THE HUMAN RESPONSE TO HAEMOPHILUS-INFLUENZAE TYPE-B CAPSULAR POLYSACCHARIDE - NUCLEOTIDE-SEQUENCES OF MONOSPECIFIC ANTI-HAEMOPHILUS ANTIBODIES AND POLYSPECIFIC ANTIBODIES CROSS-REACTING WITH SELF-ANTIGENS [J].