HIGH-RESOLUTION CRYSTALS AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF A CATALYTIC RNA

被引：16

作者：

KIM, R

HOLBROOK, EL

JANCARIK, J

PANDIT, J

WENG, XW

BOHM, A

KIM, SH

机构：

[1] LAWRENCE BERKELEY LAB,DEPT CHEM,BERKELEY,CA 94720

[2] LAWRENCE BERKELEY LAB,DIV STRUCT BIOL,BERKELEY,CA 94720

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1994年 / 50卷

关键词：

D O I：

10.1107/S0907444993013071

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-resolution single crystals of a catalytic RNA molecule derived from the sequence of the satellite RNA of tobacco ringspot virus have been obtained. The unit-cell volumes of the RNA crystals vary depending on the crystallization conditions and temperature. The best crystal form, when flash frozen, has space group P1 with unit-cell dimensions a = 53.08, b = 71.81, c = 28.03 angstrom, alpha = 98.43, beta = 104.32 and gamma = 74.540. This form diffracts to a resolution of 2.4 angstrom. A heavy-atom derivative search is in progress.

引用

页码：290 / 292

页数：3

共 9 条

[1]

BUZAYAN JM, 1986, P NATL ACAD SCI USA, V78, P6381

[2] THE CHEMISTRY OF SELF-SPLICING RNA AND RNA ENZYMES [J].