PROTECTIVE EFFECT OF GLUTATHIONE ON THE INVITRO INHIBITION OF HEPATIC CYTOCHROME-P-450 BY CAPTAN

被引:7
作者
DALVI, RR
ASHLEY, WM
机构
[1] Toxicology Laboratory Department of Physiology and Pharmacology, School of Veterinary Medicine Tuskegee Institute, Tuskegee, AL
基金
美国国家卫生研究院;
关键词
D O I
10.3109/01480547908998246
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The in vitro effect of various concentrations of captan on hepatic microsomal cytochrome P-450 from phenobarbital-pretreated rats was studied. The 1-50 value, namely the concentration of the inhibitor necessary to produce 50% loss of cytochrome P-450 was determined from the plotted inhibition curve. The presence of ethylenediaminetetraacetic acid (EDTA) in microsomal incubations prior to the addition of captan failed to prevent the loss of cytochrome P-450 by captan. In contrast, reduced glutathione (0.5 mM) added to microsomal incubations before captan (0.1 mM) afforded almost complete protection of cytochrome P-450 from captan inhibition. These data indicate that the inhibitory effect of captan on vitally important drug-metabolizing enzyme system, of which cytoehrome P-450 is a major component, can be prevented by prior presence of reduced glutathione (GSH) but not of EDTA. © 1979 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
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页码:245 / 255
页数:11
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