SEDIMENTATION-VELOCITY SEPARATION - PREPARATION METHOD FOR CERVICAL SAMPLES

被引：16

作者：

OTTO, K

HOFFKEN, H

SOOST, HJ

机构：

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1979年 / 27卷 / 01期

关键词：

D O I：

10.1177/27.1.86560

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A preparation procedure, aiming at monolayer deposition of cervical exfoliative material on glass slides for high resolution prescreening has been developed. The main features of this procedure are centrifugal deposition after suspension and sedimentation of samples over isopycnic medium of 1.026 density. Fractioning of separation of the sepaparation column after centrifugation at 50xg yields two preparations with leukocytes, bacteria and cellular debris predominantly located on the first slide and epithelial cells on the second one. The degree of spatial cellular isolation as well as the amount of diagnostically relevant cells per slide seem to fit the requirements of automated high resolution analysis.

引用

页码：14 / 18

页数：5

共 16 条

[1]

BIBBO M, 1976, AUTOMATION UTERINE C

[2]

CAMBIER MA, 1976, ACTA CYTOL, V20, P586

[3]

DANNO M, 1977, 2ND P INT C AUT CANC

[4]

FELDMAN M, 1973, ACTA CYTOL, V17, P395