PHYSICOCHEMICAL PROPERTIES OF NON-ACTIVATED AND ACTIVATED RENIN FROM HUMAN AMNIOTIC-FLUID

被引：12

作者：

ITO, T ^{[1
]}

DEVAUX, C ^{[1
]}

GAUTRAY, JP ^{[1
]}

MENARD, J ^{[1
]}

CORVOL, P ^{[1
]}

机构：

[1] INSERM, U36,17 RUE FER A MOULIN, F-75005 PARIS, FRANCE

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 569卷 / 02期

关键词：

D O I：

10.1016/0005-2744(79)90055-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The main physicochemical and enzymic properties of non-activated and activated human amniotic renin (EC 3.4.99.19) were studied in order to clarify the relationships between the two enzymes. Human amniotic renin was activated by dialysis against acidic buffer (pH 3.3), direct acidification or trypsin treatment. All procedures produced similar activation. The physicochemical characteristics of non-activated and activated renin were compared to those of human renal renin. Non-activated renin had a molecular weight of 45 500. A similar molecular weight was obtained by gel eluate activation and by acid treatment of renin prior to gel filtration. Similar isoelectric points were also found for non-activated and activated renin. One major renin peak focused at pH 6.6, whereas no similar renin peak was detected in extracts from normal human kidney. In addition, non-activated and activated renin forms were found to have the same optimal pH, same Km and the same inhibiting pepstatin concentrations. © 1979.

引用

页码：202 / 210

页数：9

共 31 条

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BARRETT JD, 1977, CIRC RES, V41, pR7

[2]

BOYD GW, 1977, LANCET, V1, P215

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