A HUMAN PUTATIVE LYMPHOCYTE G0/G1 SWITCH GENE CONTAINING A CPG-RICH ISLAND ENCODES A SMALL BASIC-PROTEIN WITH THE POTENTIAL TO BE PHOSPHORYLATED

被引:119
作者
RUSSELL, L [1 ]
FORSDYKE, DR [1 ]
机构
[1] QUEENS UNIV, DEPT BIOCHEM, KINGSTON K7L 3N6, ONTARIO, CANADA
关键词
D O I
10.1089/dna.1991.10.581
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Genes actively involved in the G0/G1 switch (G0S genes) may be differentially expressed during the lectin-induced switch of lymphocytes from the G0 to the G1 phases of the cell cycle. This paper presents studies of G0S2, a member of a set of putative G0S genes, for which cDNAs were cloned and selected on the basis of differential cDNA hybridization. G0S2 mRNA increases transiently within 1-2 hr of the addition of lectin or cycloheximide to cultured blood mononuclear cells. Comparison of a nearly full-length cDNA sequence with the corresponding genomic sequence reveals one small intron and an open reading frame in the second exon. The derived 103-amino-acid basic protein has two potential alpha-helical domains separated by a hydrophobic region with the potential to generate turns and assume a beta-sheet conformation. Consistent with involvement in the G0/G1 switch, the protein contains potential sites for phosphorylation by protein kinase C and casein kinase II. The gene contains a CpG-rich island suggesting expression in the germ line. An upstream segment contains tandem dinucleotide repeats (CT)19/(CA)16. There is a suitably located TATA box, but potential sites for CCAAT-box binding factors are far upstream, embedded in a 42-nucleotide repeat element. Potential sites for transcription factors AP1, AP2, and AP3 are consistent with rapid transcriptional activation in response to inducing agents.
引用
收藏
页码:581 / 591
页数:11
相关论文
共 53 条
[1]   CYTOKINES - COORDINATORS OF IMMUNE AND INFLAMMATORY RESPONSES [J].
ARAI, K ;
LEE, F ;
MIYAJIMA, A ;
MIYATAKE, S ;
ARAI, N ;
YOKOTA, T .
ANNUAL REVIEW OF BIOCHEMISTRY, 1990, 59 :783-836
[2]   THE MOUSE IMMUNOGLOBULIN HEAVY-CHAIN ENHANCER - EFFECT ON TRANSCRIPTION INVITRO AND BINDING OF PROTEINS PRESENT IN HELA AND LYMPHOID B-CELL EXTRACTS [J].
AUGEREAU, P ;
CHAMBON, P .
EMBO JOURNAL, 1986, 5 (08) :1791-1797
[3]  
BAEUERLE PA, 1991, MOL ASPECTS CELLULAR, V6, P423
[4]   CONTROL OF C-JUN ACTIVITY BY INTERACTION OF A CELL-SPECIFIC INHIBITOR WITH REGULATORY DOMAIN-DELTA - DIFFERENCES BETWEEN V-JUN AND C-JUN [J].
BAICHWAL, VR ;
TJIAN, R .
CELL, 1990, 63 (04) :815-825
[5]  
BERNARDI G, 1989, ANNU REV GENET, V23, P637, DOI 10.1146/annurev.ge.23.120189.003225
[6]   ACTIVATION OF RESTING HUMAN T-CELLS REQUIRES PROLONGED STIMULATION OF PROTEIN KINASE-C [J].
BERRY, N ;
ASE, K ;
KISHIMOTO, A ;
NISHIZUKA, Y .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (06) :2294-2298
[7]   HA-RAS AUGMENTS C-JUN ACTIVITY AND STIMULATES PHOSPHORYLATION OF ITS ACTIVATION DOMAIN [J].
BINETRUY, B ;
SMEAL, T ;
KARIN, M .
NATURE, 1991, 351 (6322) :122-127
[8]   3 HUMAN HOMOLOGS OF A MURINE GENE ENCODING AN INHIBITOR OF STEM-CELL PROLIFERATION [J].
BLUM, S ;
FORSDYKE, RE ;
FORSDYKE, DR .
DNA AND CELL BIOLOGY, 1990, 9 (08) :589-602
[9]   WEIGHT MATRIX DESCRIPTIONS OF 4 EUKARYOTIC RNA POLYMERASE-II PROMOTER ELEMENTS DERIVED FROM 502 UNRELATED PROMOTER SEQUENCES [J].
BUCHER, P .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 212 (04) :563-578
[10]   MITOGENIC LECTINS BIND TO THE ANTIGEN RECEPTOR ON HUMAN-LYMPHOCYTES [J].
CHILSON, OP ;
KELLYCHILSON, AE .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1989, 19 (02) :389-396