RETINOIC ACID MODULATES GAP JUNCTIONAL PERMEABILITY - A COMPARATIVE-STUDY OF DYE SPREADING AND IONIC COUPLING IN CULTURED-CELLS

被引：38

作者：

BRUMMER, F ^{[1
]}

ZEMPEL, G ^{[1
]}

BUHLE, P ^{[1
]}

STEIN, JC ^{[1
]}

HULSER, DF ^{[1
]}

机构：

[1] UNIV STUTTGART,INST BIOL,BIOPHYS ABT,PFAFFENWALDRING 57,W-7000 STUTTGART 80,GERMANY

来源：

EXPERIMENTAL CELL RESEARCH | 1991年 / 196卷 / 02期

关键词：

D O I：

10.1016/0014-4827(91)90245-P

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

All-trans retinoic acid (RA), which was recently identified as a morphogen, affects gap junctional permeability in a dose- and time-dependent manner. In five different established mammalian cell lines (FL, BRL, BICR/M1Rk, HEL37, BT5C1) 100 μmol/liter RA reduced Lucifer yellow spreading within 30 min to 20-50% of the control. Ionic coupling, however, remained almost unaffected under the same conditions. Freezefractured membranes of untreated and RA-treated cells were similar with regard to frequency and sizes of gap junction plaques. With concentrations of less than 10 μmol/liter RA the dye spreading increased significantly in the human amniotic cell line FL, pointing to a possible modulatory effect of RA on junctional communication. © 1991.

引用

页码：158 / 163

页数：6

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