ATP-INDUCED CHONDROCALCINOSIS

Objective. To determine whether adult articular cartilage mineralizes in the presence of ATP. Methods. Intact adult porcine articular cartilage and monolayers of chondrocytes were cultured in physiologic media containing ATP, and mineralization was measured as retention of Ca-45. Cartilage was analyzed by electron microscopy. Results. Articular cartilage sequestered Ca-45 when incubated with 100 muM ATP. Use of the ATP analog alpha,beta-methylene ATP did not promote mineralization and addition of pyrophosphatase inhibited mineralization, indicating that hydrolysis of ATP to AMP and inorganic pyrophosphate is necessary for the process to occur. Mineral was concentrated in articular cartilage vesicles in the perichondral area. Conclusion. Adult articular cartilage mineralizes in the presence of ATP, in a manner similar to that found with isolated matrix or articular cartilage vesicles. This supports the notion that these structures have a role in chondrocalcinosis.