INVITRO REPLICATION STUDY OF MODIFIED BASES IN RAS SEQUENCES

被引：52

作者：

KAMIYA, H

SAKAGUCHI, T

MURATA, N

FUJIMURO, M

MIURA, H

ISHIKAWA, H

SHIMIZU, M

INOUE, H

NISHIMURA, S

MATSUKAGE, A

MASUTANI, C

HANAOKA, F

OHTSUKA, E

机构：

[1] HOKKAIDO UNIV, FAC PHARMACEUT SCI, KITA-12 NISHI-6, KITA KU, SAPPORO, HOKKAIDO 060, JAPAN

[2] NATL CANC CTR, RES INST, DIV BIOL, CHUO KU, TOKYO 104, JAPAN

[3] AICHI CANC CTR, RES INST, CELL BIOL LAB, CHIKUSA KU, NAGOYA, AICHI 464, JAPAN

[4] RIKEN INST PHYS & CHEM RES, CELLULAR PHYSIOL LAB, WAKO, SAITAMA 35101, JAPAN

来源：

CHEMICAL & PHARMACEUTICAL BULLETIN | 1992年 / 40卷 / 10期

关键词：

O-6-METHYLGUANINE; 8-HYDROXYGUANINE; XANTHINE; HYPOXANTHINE; TAQ DNA POLYMERASE; DNA POLYMERASE-BETA; DNA POLYMERASE-ALPHA; REPLICATION; RAS GENE;

D O I：

10.1248/cpb.40.2792

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

DNA templates containing a modified base (O6-methylguanine, 8-hydroxyguanine, xanthine or hypoxanthine) which was located in nucleotide sequences corresponding to the 12th or 61st codon of a ras gene were synthesized and deoxynucleotide incorporation opposite the lesions was investigated. The templates were replicated by Taq DNA polymerase, recombinant rat DNA polymerase beta and mouse DNA polymerase alpha-primase complex. Sequence analysis of the replicated products indicated selective incorporation of nucleotide(s) opposite a modified base, depending on the kind of base and of DNA polymerase. This system is very useful to obtain results of in vitro replication of modified bases in ras sequences.

引用

页码：2792 / 2795

页数：4

共 27 条

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