IMPROVED INVITRO LIGHT ACTIVATION AND ASSAY SYSTEMS FOR 2 SPINACH CHLOROPLAST ENZYMES

被引：41

作者：

SCHURMANN, P ^{[1
]}

JACQUOT, JP ^{[1
]}

机构：

[1] UNIV NANCY 1,CNRS,BIOL VEGETALE LAB,EQUIPE RECH 799,F-54037 NANCY,FRANCE

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 569卷 / 02期

关键词：

Chloroplast enzyme; Fructose-1,6-bisphosphatase; Light activation; NADP-dependent malate dehydrogenase; Thioredoxin;

D O I：

10.1016/0005-2744(79)90067-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An improved system for the in vitro light activation of the chloroplast enzymes fructose-1,6-bisphosphatase and NADP-dependent malate dehydrogenase is described. Through the presence of the monothiol β-mercapto-ethanol in the reaction mixtures the activated forms of the enzymes can be stabilized and their activity determined spectrophotometrically. © 1979.

引用

页码：309 / 312

页数：4

共 6 条

[1] EVIDENCE FOR EXISTENCE OF SEVERAL ENZYME-SPECIFIC THIOREDOXINS IN PLANTS [J].

JACQUOT, JP ;

VIDAL, J ;

GADAL, P ;

SCHURMANN, P .

FEBS LETTERS, 1978, 96 (02) :243-246

[2] IDENTIFICATION OF A PROTEIN FACTOR INVOLVED IN DITHIOTHREITOL ACTIVATION OF NADP MALATE-DEHYDROGENASE FROM FRENCH BEAN-LEAVES [J].

JACQUOT, JP ;

VIDAL, J ;

GADAL, P .

FEBS LETTERS, 1976, 71 (02) :223-227

[3] STUDIES ON REGULATORY PROPERTIES OF CHLOROPLAST FRUCTOSE-1,6-BISPHOSPHATASE [J].

SCHURMANN, P ;

WOLOSIUK, RA .

BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 522 (01) :130-138

[4]

WOLOSIUK RA, 1979, J BIOL CHEM, V254, P1627

[5] REGULATION OF NADP-MALATE DEHYDROGENASE BY LIGHT-ACTUATED FERREDOXIN-THIOREDOXIN SYSTEM OF CHLOROPLASTS [J].

WOLOSIUK, RA ;

BUCHANAN, BB ;

CRAWFORD, NA .

FEBS LETTERS, 1977, 81 (02) :253-258

[6] EFFICIENT PURIFICATION AND MOLECULAR-PROPERTIES OF SPINACH CHLOROPLAST FRUCTOSE 1,6-BISPHOSPHATASE [J].

ZIMMERMANN, G ;

KELLY, GJ ;

LATZKO, E .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1976, 70 (02) :361-367

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