EFFECT OF DETERGENT ON PUROMYCIN-MEDIATED RELEASE OF NASCENT PEPTIDES FROM MAMMALIAN LIVER RIBOSOMES

被引：11

作者：

NABI, N

ODA, T

OMURA, T

机构：

[1] Department of Biology, Faculty of Science, Kyushu University, Fukuoka

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 87卷 / 03期

关键词：

D O I：

10.1016/0006-291X(79)92022-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Puromycin-mediated in vitro release of nascent peptides from free and bound ribosomes prepared from rat livers was markedly enhanced by the presence of a non-ionic detergent, Triton X-100, in the incubation medium, and the enhancement by the detergent was more pronounced for bound ribosomes than for free ribosomes. Same results were obtained when nascent peptides were labeled either in vivo by radioactive amino acids or in vitro by radioactive puromycin. © 1979.

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页码：750 / 756

页数：7

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