EFFECT OF TUNICAMYCIN ON MOLECULAR HETEROGENEITY OF COLONY STIMULATING FACTOR IN CULTURED MOUSE MAMMARY CARCINOMA-FM3A CELLS

被引：19

作者：

AYUSAWA, D ^{[1
]}

ISAKA, K ^{[1
]}

SENO, T ^{[1
]}

TOMIDA, M ^{[1
]}

YAMAMOTO, Y ^{[1
]}

HOZUMI, M ^{[1
]}

TAKATSUKI, A ^{[1
]}

TAMURA, G ^{[1
]}

机构：

[1] SAITAMA CANC CTR, DEPT CHEMOTHERAPY, SAITAMA, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 90卷 / 03期

关键词：

D O I：

10.1016/0006-291X(79)91896-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Granulocyte and macrophage colony-stimulating factors obtained from cultured mouse mammary carcinoma FM3A cells showed heterogeneity in molecular size giving rise to a major component with an apparent MW of 80,000 and a minor one with that of 35,000 on Sephadex G-200 column chromatography. In the presence of tunicamycin, a specific inhibitor of asparagine-linked glycosylation, the colony-stimulating factor was produced normally and consisted of a single component with an apparent MW of 30,000. These data indicate that the sugar moiety is not essential for the production or activity of colony-stimulating factor and that the heterogeneity in molecular size of the colony-stimulating factor mainly resulted from tunicamycin-sensitive glycosylation.

引用

页码：783 / 787

页数：5

共 19 条

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