PRO-OXIDATIVE INTERACTIONS OF DITHRANOL WITH HUMAN PHAGOCYTES PROMOTE OXIDATIVE DAMAGE TO DNA OF BYSTANDER LEUKOCYTES

被引：4

作者：

FORRESTER, DMT ^{[1
]}

VANRENSBURG, CEJ ^{[1
]}

ANDERSON, R ^{[1
]}

机构：

[1] UNIV PRETORIA,INST PATHOL,DEPT IMMUNOL,MRC UNIT STUDY PHAGOCYTE FUNCT,POB 2034,PRETORIA 0001,SOUTH AFRICA

来源：

MUTATION RESEARCH | 1991年 / 247卷 / 01期

关键词：

DITHRANOL; DNA STRAND BREAKS; NEUTROPHILS; LYMPHOCYTES; OXIDANTS;

D O I：

10.1016/0027-5107(91)90031-I

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Dithranol at therapeutic concentrations (5-40-mu-g ml-1) induced strand breaks in human leucocyte DNA in vitro in a dose-related manner. Leucocytes from individuals with chronic granulomatous disease (CGD) incurred substantially less DNA strand breaks than did normal leucocytes during exposure to dithranol indicating that activated phagocytes are involved. H-7, 4-beta-bromophenacyl bromide (BPB) and staurosporine, all inhibitors of protein kinase C, decreased both dithranol-mediated activation of the phagocyte respiratory burst and induction of DNA strand breaks. Similar effects were observed with the hydrogen peroxide scavenger catalase. These results suggest that dithranol induces DNA strand breaks, mainly as a result of pro-oxidative interactions with phagocytes.

引用

页码：39 / 44

页数：6

共 20 条

[1] DITHRANOL MEDIATES PRO-OXIDATIVE INHIBITION OF POLYMORPHONUCLEAR LEUKOCYTE MIGRATION AND LYMPHOCYTE-PROLIFERATION [J].