A SPECTROELECTROCHEMICAL STUDY OF MICROPEROXIDASE AT BARE AND GOLD-PLATED RVC THIN-LAYER ELECTRODES

被引：25

作者：

ZAMPONI, S

SANTUCCI, R

BRUNORI, M

MARASSI, R

机构：

[1] UNIV ROME LA SAPIENZA,DEPT BIOCHEM SCI,PLE ALDO MORO 5,I-00185 ROME,ITALY

[2] UNIV CAMERINO,DEPT CHEM,I-62032 CAMERINO,ITALY

[3] UNIV ROME LA SAPIENZA,CNR,CTR MOLEC BIOL,I-00185 ROME,ITALY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1034卷 / 03期

关键词：

Active site; Microperoxidase; Residual vitrous carbon; RVC thin-layer electrode; Spectroelectrochemistry;

D O I：

10.1016/0304-4165(90)90054-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Direct electrochemistry of microperoxidase (the heme-undecapeptide from cytochtome) has been followed at a bare and a gold plated RVC thin-layer electrode, using the spectropotentiostatic method or voltabsorptometry. Both techniques yield 'clean' and undistorted signals; their analysis easily provides quantitative information for the electrochemical parameters of microperoxidase and shows that spectroelectrochemistry is a powerful method to study the redox behavior of metalloproteins or their active site fragments. © 1990.

引用

页码：294 / 297

页数：4

共 15 条

[1] MECHANISM OF THE REDUCTION AND OXIDATION REACTION OF CYTOCHROME-C AT A MODIFIED GOLD ELECTRODE [J].