IDENTIFICATION AND CHARACTERIZATION OF CDNA CLONES ENCODING CINNAMYL ALCOHOL-DEHYDROGENASE FROM TOBACCO

被引：91

作者：

KNIGHT, ME ^{[1
]}

HALPIN, C ^{[1
]}

SCHUCH, W ^{[1
]}

机构：

[1] ICI SEEDS,PLANT BIOTECHNOL SECT,JEALOTTS HILL RES STN,BRACKNELL RG12 6EY,ENGLAND

来源：

PLANT MOLECULAR BIOLOGY | 1992年 / 19卷 / 05期

关键词：

LIGNIN; CAD; DNA SEQUENCE; TOBACCO;

D O I：

10.1007/BF00027075

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cinnamyl alcohol dehydrogenase (CAD, EC 1.1.1.195) is an enzyme involved in lignin biosynthesis. We have previously isolated pure CAD enzyme as two closely related polypeptides of 44 and 42.5 kDa from tobacco stems. In this paper, we report partial amino acid sequences of these two polypeptides. Based on the peptide sequences mixed oligonucleotides were used to screen a tobacco stem cDNA library and CAD cDNA clones encoding the two polypeptides were identified. DNA sequence comparisons indicate very high sequence identity between these clones both in the coding and in the 5' and 3' untranslated sequences. The close similarity between the two CAD genes leads us to suggest that they do not represent different isoforms but are the same gene from each of the two parental lines of Nicotiana tabacum cv. Samsun. Sequence comparisons with alcohol dehydrogenase 1 (ADH1) from yeast shows sequence similarities of ca. 30%, while comparisons with maize, barley and potato ADH1 sequences show similarities of not more than 23%.

引用

页码：793 / 801

页数：9

共 21 条

[1] PURIFICATION OF BIOLOGICALLY-ACTIVE GLOBIN MESSENGER-RNA BY CHROMATOGRAPHY ON OLIGOTHYMIDYLIC-ACID-CELLULOSE [J].

AVIV, H ;

LEDER, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1972, 69 (06) :1408-&

[2]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[3]

DENNIS ES, 1984, NUCLEIC ACIDS RES, V12, P3982

[4] RAPID TRANSIENT INDUCTION OF PHENYLALANINE AMMONIA-LYASE MESSENGER-RNA IN ELICITOR-TREATED BEAN CELLS [J].

EDWARDS, K ;

CRAMER, CL ;

BOLWELL, GP ;

DIXON, RA ;

SCHUCH, W ;

LAMB, CJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (20) :6731-6735

[5] AN ASPARTATE RESIDUE IN YEAST ALCOHOL DEHYDROGENASE-I DETERMINES THE SPECIFICITY FOR COENZYME [J].

FAN, F ;

LORENZEN, JA ;

PLAPP, BV .

BIOCHEMISTRY, 1991, 30 (26) :6397-6401

[6] THE C-TERMINAL DOMAIN OF PLANT CATALASES - IMPLICATIONS FOR A GLYOXYSOMAL TARGETING SEQUENCE [J].

GONZALEZ, E .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 199 (01) :211-215

[7] NUCLEOTIDE-SEQUENCE OF A COMPLETE BARLEY ALCOHOL DEHYDROGENASE-1 CDNA [J].

GOOD, AG ;

PELCHER, LE ;

CROSBY, WL .

NUCLEIC ACIDS RESEARCH, 1988, 16 (14) :7182-7182

[8] COLONY HYBRIDIZATION - METHOD FOR ISOLATION OF CLONED DNAS THAT CONTAIN A SPECIFIC GENE [J].

GRUNSTEIN, M ;

HOGNESS, DS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (10) :3961-3965

[9] PURIFICATION AND CHARACTERIZATION OF CINNAMYL ALCOHOL-DEHYDROGENASE FROM TOBACCO STEMS [J].

HALPIN, C ;

KNIGHT, ME ;

GRIMAPETTENATI, J ;

GOFFNER, D ;

BOUDET, A ;

SCHUCH, W .

PLANT PHYSIOLOGY, 1992, 98 (01) :12-16

[10]

Jepson I., 1991, PLANT MOL BIOL REP, V9, P131

← 1 2 3 →