DEPURINATION OF PLANT RIBOSOMES BY POKEWEED ANTIVIRAL PROTEIN

被引：56

作者：

TAYLOR, BE ^{[1
]}

IRVIN, JD ^{[1
]}

机构：

[1] SW TEXAS STATE UNIV,DEPT CHEM,SAN MARCOS,TX 78666

来源：

FEBS LETTERS | 1990年 / 273卷 / 1-2期

关键词：

Depurination; Plant ribosome; Pokeweed antiviral protein; Ribosome inactivating protein;

D O I：

10.1016/0014-5793(90)81070-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mammalian ribosomes have been shown to be enzymatically modified by ribosomal inactivating protein (RIPs) via specific depurination of rRNA. Here we report that ribosomes isolated from wheat germ contain intact and undepurinated rRNA and are depurinated by pokeweed antiviral protein (PAP). Pokeweed ribosomes isolated under the same conditions are depurinated. Total RNA isolated from pokeweed in the presence of strong denaturants was found to pbe partially depurinated. We conclude that wheat germ ribosomes are resistant to the endogenous RIP, tritin, but are sensitive to PAP and that pokeweed ribosomes can be depurinated by the N-glycosidase activity of endogenous PAP during isolation. © 1990.

引用

页码：144 / 146

页数：3

共 21 条

[1]

BARBIERI L, 1982, CANCER SURV, V1, P489

[2] DIFFERENTIAL EFFECT OF RIBOSOME-INACTIVATING PROTEINS ON PLANT RIBOSOME ACTIVITY AND PLANT-CELLS GROWTH [J].