CHARACTERIZATION OF MUSCLE AND NONMUSCLE XENOPUS-LAEVIS TROPOMYOSIN MESSENGER-RNAS TRANSCRIBED FROM THE SAME GENE - DEVELOPMENTAL AND TISSUE-SPECIFIC EXPRESSION

被引:35
作者
HARDY, S
FISZMAN, MY
OSBORNE, HB
THIEBAUD, P [1 ]
机构
[1] FAC SCI BEAULIEU,BIOL & GENET DEV LAB,CNRS,URA 256,AV GAL LECLERC,F-35042 RENNES,FRANCE
[2] INST PASTEUR,DEPT BIOL MOLEC,F-75724 PARIS 15,FRANCE
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1991年 / 202卷 / 02期
关键词
D O I
10.1111/j.1432-1033.1991.tb16392.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have isolated and characterized three XenopUs laevis tropomyosin cDNAs (XTM-alpha-2, XTM-alpha-4 and XTM05). XTM-alpha-2 and XTM-alpha-4 were isolated from a stage-42 embryo cDNA library and XTM05 from an oocyte cDNA library. XTM-alpha-2 and XTM-alpha-4 both code for a 284-amino-acid protein homologous to the skeletal alpha-tropomyosin but diverge in their 3' untranslated sequences. This divergence is due to an absence of splicing in the early embryo. XTM05 codes for a 248-amino-acid non-muscle tropomyosin. Sequence analysis indicates that the three cDNAs are generated from a single gene which uses two promoters and possibly three sets of alternate exons. Northern blot analysis, with specific probes against each cDNA, was used to study the expression of the X. laevis-alpha tropomyosin gene during development and in specific tissues. This analysis showed a different temporal usage of the two promoters and characterized the tissue-specific expression of the different transcripts.
引用
收藏
页码:431 / 440
页数:10
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