EXPRESSION OF ELONGATION FACTOR-I GAMMA-RELATED SEQUENCE IN HUMAN PANCREATIC-CANCER

被引：48

作者：

LEW, Y ^{[1
]}

JONES, DV ^{[1
]}

MARS, WM ^{[1
]}

EVANS, D ^{[1
]}

BYRD, D ^{[1
]}

FRAZIER, ML ^{[1
]}

机构：

[1] UNIV TEXAS,MD ANDERSON CANC CTR,DEPT MED ONCOL,HOUSTON,TX 77030

来源：

PANCREAS | 1992年 / 7卷 / 02期

关键词：

ELONGATION FACTOR-1-GAMMA; PANCREATIC CANCER;

D O I：

10.1097/00006676-199203000-00003

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

A cDNA clone designated pPDC-1 was isolated from a cDNA library prepared against poly(A +)RNA isolated from the human pancreatic adenocarcinoma cell line, Capan-2. The cDNA corresponds to a 1.7-kilobase mRNA that is expressed at higher levels in seven of nine pancreatic tumors than in their corresponding normal tissues. It is also expressed in normal human kidney, intestine, pancreas, stomach, placenta, lung, brain, spleen, and liver. A computer search of the Intelligenetics System of all available nucleotide sequences revealed a 60% homology between the nucleotide sequence of the pPDC-1 cDNA and that of elongation factor-1 gamma from Artemia. The deduced amino acid sequence shared 53% identity with the amino acid sequence for the Artemia elongation factor-1 gamma.

引用

页码：144 / 152

页数：9

共 30 条

[1]

Cubilla A.L., Fitzgerald P.J., Cancer of the pancreas (Nonendocrine): A suggested morphologic classification, Semin Oncol, 6, pp. 285-297, (1979)

[2]

Maessen G., Amons R., Zeelen J.P., Moller W., Primary structure of elongation factor-1 gamma from Artemia, FEBS Lett, 223, pp. 181-186, (1987)

[3]

Miller D.L., Weisbach H., Factors involved in the transfer of amino acyl-tRNA to the ribosome, Molecular Mechanisms of Protein Biosynthesis, pp. 323-373, (1977)

[4]

Frazier M.L., Pathak S., Wang Z.W., Cleary K., Singletary S.E., Olive M., Mackay B., Steck P.A., Levin B., Establishment of a new human pancreatic adenocarcinoma cell line, MDAPanc-1, Pancreas, 5, pp. 8-16, (1990)

[5]

Frazier M., Mars W., Florine D., Montagna R., Saunders G., Efficient extraction of RNA from mammalian tissue, Mol Cell Biochem, 56, pp. 113-122, (1983)

[6]

Aviv H., Leder P., Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose, Proc Natl Acad Sci USA, 69, pp. 1408-1412, (1972)

[7]

Kahn M., Kolter R., Thomas C., Et al., Plasmid cloning vehicles derived from plasmids Col El, F, R6K, and RK2, Methods in Enzymology, 68, pp. 268-280, (1979)

[8]

Radloff R., Bauer W., Vinograd J., A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: The closed circular DNA in Hela cells, Proc Natl Acad Sci USA, 57, pp. 1514-1521, (1967)

[9]

Kraft R., Tardiff J., Krauter K.S., Leinwand L.A., Using miniprep plasmid DNA for sequencing double stranded templates with sequenase, Biotechniques, 6, pp. 544-547, (1988)

[10]

Sanger F., Coulson A., Barrell B., Smith A., Roe B., Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing, J Mol Biol, 143, pp. 161-178, (1980)

← 1 2 3 →