MECHANISM OF INHIBITORY-ACTION OF CAPSAICIN ON PARTICULATE AXOPLASMIC-TRANSPORT IN SENSORY NEURONS IN CULTURE

被引:24
作者
KAWAKAMI, T
HIKAWA, N
KUSAKABE, T
KANO, M
BANDOU, Y
GOTOH, H
TAKENAKA, T
机构
[1] YOKOHAMA CITY UNIV,SCH MED,DEPT ANAT,KANAZAWA KU,YOKOHAMA,KANAGAWA 232,JAPAN
[2] IWATE MED UNIV,SCH MED,DEPT PHYSIOL,MORIOKA,IWATE 020,JAPAN
来源
JOURNAL OF NEUROBIOLOGY | 1993年 / 24卷 / 05期
关键词
CAPSAICIN; AXOPLASMIC TRANSPORT; DORSAL ROOT GANGLION CELLS; C-FIBER; FURA-2;
D O I
10.1002/neu.480240502
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The inhibitory effect Of capsaicin on axoplasmic transport in cultured dorsal root ganglion cells was analyzed by video-enhanced contrast microscopy. Capsaicin inhibited particle transports in a dose-dependent manner, irrespective of the diameter of axons. The effect of capsaicin was reversible at low concentrations. Capsaicin affected both the anterograde and retrograde transport. Large organelles were more sensitive to capsaicin than small ones in the retrograde transport. An experiment using calcium-sensitive dye, Fura 2, indicated that capsaicin raised the intraneuronal free calcium concentration preceding the inhibition of the transport. Electron microscopy revealed that microtubules and neurofilaments are disorganized and disoriented by capsaicin. We reached a conclusion that capsaicin inhibits fast axoplasmic transport of both anterograde and retrograde directions in all types of somatosensory neurons in culture by disorganizing intraaxonal cytoskeletal structures, through the elevated intracellular Ca2+ concentration.
引用
收藏
页码:545 / 551
页数:7
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